Label-free detection methods and electrical impedance measurements are becoming more and more accepted as methods for monitoring cells in vitro, as seen at this year´s Screening Europe, held on 20-21 February in Barcelona, Spain.
The impedance technology of the Bionas® 2500 analyzing system differs from other instruments launched over the past few years due to additional, label-free measurements of acidification and oxygen consumption rates of living cells. The system has an automatic fluidics device, which supplies the cells at regular, programmable intervals with fresh medium or medium with the test substances. The device is well suited for long-term observations of living cells under conditions close to in vivo and for observing recovery effects. The perfusion system ensures there is no accumulation of interfering metabolites.
While some impedance measurement instruments are designed for well-based screening applications, the Bionas® 2500 focuses on high-content analysis. “Discussions at Screening Europe have confirmed our assumption that high-content analysis is valued more highly than throughput in lead discovery,” says Dr. Michael Schulze, Head of Marketing and Sales at Bionas. “Our technology represents a holistic approach providing deeper insights and allowing earlier and faster selection of promising drug candidates while at the same time reducing time and costs in drug discovery.”
About Bionas – www.bionas.de
Bionas GmbH, located in Rostock, Germany, specializes in analysis systems and services for profiling the metabolic activity of cells in vitro to understand cellular function. The Bionas® 2500 analyzing system measures extracellular acidification, oxygen consumption and cell adhesion in a label-free and non-invasive manner and can be applied to various cell types including primary cells. The readout is continuous and can be watched online. Main applications include drug profiling, lead optimization, pharmacokinetics, early toxicology programs, ADME/Tox, chemosensitivity testing, toxicological testing of chemical substances (REACH) and cell culture monitoring/optimization. Micronas Holding GmbH and GENIUS Venture Capital GmbH fund the company.
Impedance is based on the insulating effect of intact cell membranes on sensor electrodes: this determines the current flow and thus the sensor signal. Measuring impedance monitors cellular adhesion and confluence, which is useful for research and screening purposes, e.g. to screen for GPCRs and their modulators, in cancer research and to test cell viability.
Contact at Bionas GmbH:
Dr. Michael Schulze
Tel.: +49 (0) 381 5196-241